Luminometer promega


5 attomole) of ATP using the Promega ENLITEN® ATP Assay CONCLUSION The Veritas™ Microplate Luminometer offers superior sensitivity and dynamic range for luminescence-based ATP assay. # A7699 or GE Healthcare Cat. # 27-1006) Luminometers Note: t is suggested to use a dedicated luminometer when running MycoAlert™ Assays. 3M A. Promega manufactures kits for colorimetric, fluorescent, or luminescent-based measurements of cell viability, all of which can be performed in a homogeneous format (adding the reagent directly to cultures in 96-well or 384-well plates). C; Promega Cat. The luminescence module can detect as little as 3 × 10–21 moles of luciferase, covering a dynamic range over 8 logs. Compact Cells were lysed with passive lysis buffer and the luciferase assay was performed using the firefly-luciferase reporter assay system (Promega) and an MLX luminometer (Dynex technology) Sep 20, 2017 · Promega Glomax Multi Detection Plate Reader available from The Lab World Group. Read More About Sensitivity Luminometers are essential for interpreting experimental results. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. Connecting the Optocomp to a Personal Computer (249 KB) Details interfacing the Optocomp luminometers to a personal computer and capturing Optocomp data on the personal computer. 1 This test method covers the measurement of the flame radiation characteristics of aviation turbine fuels and other similar distillate fuels expressed in terms of luminometer numbers. Louis, MO, USA) as a substrate. My assay was done with HEK293 cells which were stimulated with Etoposide. For manual luminometers, the substrate solution is mixed by hand with the cell lysate, and the fluorescence is read at a defined time following mixing. E5321 - GloMax 20/20 Luminometer w/Single Auto-Injector, 1 Each - GloMax 20/20 Luminometer w/Single Auto-Injector, Promega - Each: Amazon. Promega Corporation Recommended for you. . ATP is a molecule found only in and around living cells, and as such it gives a direct measure of biological concentration and health. GloMax® Navigator is an easy-to-use microplate luminometer integrated with Promega chemistries for superior luminescence assay performance. Jun 24, 2020 · Internal ribosome entry sites (IRESs) allow the translation of a transcript independent of its cap structure. I performed a dual luciferase assay using pGL3 firefly luc as experimental reporter and pRL-TK renilla luc as internal control (Promega assay kit). 2. (dissolved substrate is stable for 1 mo. A mixture of Trypsin and rLys-C designed to improve digestion of proteins or protein mixtures in solution. 1 ng ATP or more than 4 orders of magnitude (Figure 1). , Ltd. We studied 37 The resulting cell lysates were assayed for luciferase activity using Luciferase Assay Kit (Promega, Madison, WI, USA) and TD-20/20 Luminometer (Turner Designs, Sunnyvale, CA, USA). If you are performing single assays with a single tube luminometer, this is not an issue. Transcriptional regulation, coupled to the expression of a luciferase reporter gene, is Veritas™ Microplate Luminometer detects as little as 1. com/pro Firefly and Renilla luciferase activities were assayed 48 h after transfection using a Dual Glo Luciferase Assay System (Promega) with a luminometer (Enspire, PerkinElmer) Statistical analysis For all statistical analyses not described elsewhere, we utilised Prism software (GraphPad Software). Cell health assays are used to detect and monitor various cellular events and build a picture of how and why cells live or die. “glow”. Some labs require this additional verification procedure. GloMax® Discover System. promega. Promega Corporation is a worldwide leader in applying biochemistry and molecular biology to the development of innovative, high-value products for the life sciences. Designed to work seamlessly with Promega assays, GloMax® microplate readers and luminometers come preloaded with protocols that allow you to run assays at the touch of a button. These accessories are only for the Turner BioSystems Luminometer Model TD-20/20. This sensitive instrument is used to measure luminescence. Description. In addition, the assay solution itself, as well as the conditioned media (i. Pivotal players studied in the Luminometer report: Promega Berthold Technologies Charm Sciences Lonza St. Jun 03, 2020 · A portion of the lysate is mixed with the Luciferase Assay Reagent, and luminescence measured with a single-tube luminometer, like the GloMax® 20/20 Instrument. When a luminescence reaction is set up in a microplate, a luminometer (or luminescence microplate reader), is used to measure the amount of light produced. The GloMax® 20/20 Luminometer is an ultrasensitive, versatile and affordable luminometer designed for use with any Promega bioluminescent assay. Luminescence Module:  An advanced head-on photon-counting photomultiplier tube (PMT) provides unmatched signal-to-noise ratios, beating most standalone luminometers. Luminoskan. We maintain four instruments for real time quantitative PCR. Literature # TM040. For assays that require shaking, three mixing modes are available: linear, orbital and cross shaking. A Veritas™ Microplate Luminometer Method for Promega Dual-Glo™ Luciferase 1. # Lactate-Glo™ Assay 5ml J5021 The intensity of light emission is linearly related to the amount of luciferase and is measured using a luminometer. The Microplate Luminometer Centro XS³ LB 960 is a high-sensitivity reader able to detect 1. Typically, a promoter region containing 2 to 3 kb of a 5′ flanking sequence and part of the first exon is cloned in a commercial vector PGL3‐basic vector (Promega) to obtain a photinus luciferase gene driven by the promoter of the luminometer. Promega Buys Turner Biosystems Aug 03, 2009 NEW YORK (GenomeWeb News) – Life sciences products company Promega has purchased Turner Biosystems for an undisclosed sum, Promega said today. I If using a multi-functional reader, please be sure to check the sensitivity of the device using the MycoAlert™ Assay Control Set. Product Components and Storage Conditions PRODUCT SIZE CAT. Run a variety of luminometric research applications with the Thermo Scientific™ Luminoskan™ Ascent Microplate Luminometer, a compact and robust instrument offering versatile plate formats, fast reading speeds and up to three dispensers. Biorad CFX 96, CFX Connect x 3. ZERO BIAS - scores, article reviews, protocol conditions and more The GloMax® 20/20 Luminometer is designed to provide ultra-high performance for bioluminescent and chemiluminescent assays. Ultrasensitive Single Tube Luminometer Two fluorescence Promega GloMax™-Multi Detection System: Luminescence Module An advanced photon-counting photomultiplier tube (PMT) provides unmatched signal-to-noise ratios, beating most standalone luminometers. Skip navigation Sign in. The luciferase assay is a very sensitive and convenient way to examine the transcriptional activity of a gene. Luminometers are central to all luminescence studies and in the past, journals Luminoskan Microplate Luminometer Luminoskan Microplate Luminometer, 100–240 V, 50/60 Hz* 5300330 Luminoskan Microplate Luminometer, 100–240 V, 50/60 Hz, with one dispenser* 5300331 Luminoskan Microplate Luminometer, 100–240 V, 50/60 Hz, with two dispensers* 5300332 Fluoroskan FL Microplate Fluorometer and Luminometer Hello all, I recently purchased a Caspase 3/7 Glo assay kit from Promega for apoptosis detection. 4:39. See promotional offers below The GloMax® 96 Microplate Luminometer represents instrumentation and software in a complete solution that includes bioluminescent assays, protocols and support. A number new products have been launched in the recent years. These instruments offer high sensitivity, pre-loaded protocols, and integrated data analysis—giving researchers a simple way to get better data and connect to manual or automated laboratory workflows. It allows the user to check the reproducibility, sensitivity, and linearity of the luminometer. The luminescence readings are expressed as RLU. MATERIALS Luminometer Market Analysis, Size, Share and Forecast 2020-2026 – Market Research Report | Promega, Berthold Technologies, Charm Sciences June 2, 2020 [email protected] Latest Market Research on Global Luminometer Market with Industry Assessment, Trends, Competitive Landscape, Investment Strategies and Forecast by 2026. Measurements are linear from 760 fg to 5. PLoS ONE Alerts: New Articles Orion II is a high quality microplate luminometer for 96 and 384 well plates. GloMax 96 Microplate Luminometer with Dual Reagent Injectors - Reporter Assays, Promega : Dual-Glo Luciferase Assay System: A homogeneous reagent system that allows quantitation of stable luminescence from two reporter genes in a single sample. Promega GloMax Luminescence Module has a dual masking system which minimizes well-to-well cross-talk. 3. Operating ATP assays: ATP is present in all living cells and is needed in the reaction of luciferin with luciferase to produce light, so it is not surprising that luminescence-based ATP assays are often used to quantify living cells, for example, to asses cell viability, cell proliferation or biomass. This instrument has passed validation at Promega Corporation to b The GloMax®-20/20 Luminometer Light Standard, Cat #E5341, provides a quick and easy way to verify the performance of the GloMax®-20/20 Luminometer. It features two optional injectors and reads both glow- and flash-luminescent reactions in 96-well plates. , Luminex Corporation, STRATEC SE, SAFAS and MicroDigital Co. It is the instrument of choice for Reporter Gene microplate luminometer Centro LB 960 The GloMax®-20/20 Luminometer Light Standard provides an external control to verify performance. 0 Fluorescence Reader for DNA, RNA and Protein (user-operated) Promega GloMax GM2000 Manuals Manuals and User Guides for Promega GloMax GM2000. Description: The GloMax® navigator is an easy-to-use microplate luminometer integrated with Promega chemistries for superior luminescence assay performance. The instrument is designed for measuring single-tube assays. If using a multi-functional reader, please be sure to check the sensitivity of the device using the MycoAlert™ Assay Control Set. e. 5 zmol Firefly luciferase per well. The luminometers with injection systems are available for use with dual-reporter assays like the Dual-Luciferase systems. This is easily done by assaying serial dilutions of a sample. They are not for the Turner BioSystems 20/20n Single-Tube Luminometer or Promega GloMax® 20/20 Luminometer. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. The reason for this is simple, but can be confusing to new users. The ADP-Glo™ Kinase assay is a universal, homogeneous, high-throughput screening method to measure kinase activity by quantifying the amount of ADP produced during the kinase reaction. Thermo Scientific ™ Fluoroskan ™, Fluoroskan ™ FL, and Luminoskan ™ microplate readers are compact , robust, and high-quality products with excellent performance. For Research Use Only. • luminometer, CCD camera or imaging device capable of reading luminescence in multiwell plates • optional: ATP for use in generating a standard curve (see Section 3. The touchscreen interface provides comprehensive instrument control and data collection. GM3000. The Microplate Luminometer Centro XS³ LB 960 is a high-sensitivity reader able to detect <1. Reading the light standard before taking measurements is a quick and easy way to ensure quality control of reproducibility, sensitivity, and linearity of measurements. Luminometers Promega GloMax™-Multi Jr Single-Tube Multimode Reader: Absorbance Filter Paddle The GloMax-Multi Jr Single-Tube Multimode Reader is designed to provide the utmost flexibility. # P1132, Sigma Cat. The Promega mission statement is: To be the most responsive supplier of biological reagents and reagent systems used in research and applied technology applications worldwide. com FREE SHIPPING on qualified orders DUAL LUCIFERASE REPORTER SYSTEM (PROMEGA) (24 well plate) Transfections Cells should be transfected with a test reporter (firefly luciferase) and a normalization reporter driven by a constitutive promoter (renilla luciferase) PRL-TK (thymidine kinase promoter) works well at a ratio of 1:200. Promega GloMax 20/20 Luminometer: Replacement Tubing, Valves, Tips Replacement Tubing, Valves and Tips Spectrophotometers, Refractometers and Benchtop Instruments:Luminometers Available with up to two reagent injectors, the Veritas™ Luminometer is a versatile system designed to perform both flash- and glow-type luminescence assays. For use with GloMax™ 20/20 Luminometer, GloMax-Multi Jr Single-Tube Multimode Reader,  Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA GloMax™ 20/20 Luminometer with Single Auto-Injector 1 instrument. This is an unused Promega GloMax 96 Highly Sensitive Microplate Luminometer with Software. Specifically, the GloMax® 20/20 Luminometer accommodates mea Home » Products » Spectrometer - Plate Reader » Luminometer » Promega GloMax 96 Microplate Luminometer Advanced search Keyword(s): Manufacturer: ALL (AIMS) Animal Identification & Marking Systems Inc. at –70°C) 3. I primarily use the luminometer for luciferase assays using Promega’s Dual Luciferase Kit. Jun 07, 2019 · Is this just for firefly, Renilla, or NanoLuc® luciferase assay or this is the rule for any luciferase assay. The GloMax 20/20 Luminometer is an ultrasensitive, versatile and affordable luminometer designed for use with any Promega bioluminescent assay. To reserve time see our Reservation and Billing site. Description: 1. In addition to high performance, the GloMax® 20/20 blends user Promega GloMax™ 96 Microplate Luminometers Achieve high sensitivity and broad dynamic range. com: Industrial & Scientific Useful for a variety of luminometric research applications, the Thermo Scientific Luminoskan Microplate Luminometer is a compact and robust instrument offering plate format versatility, fast reading speeds, and up to three dispensers. Additionally, the GloMax™ 20/20 Luminometer will measure chemiluminescence from both horseradish peroxidase and alkaline phosphatase. E6521: GloMax 96 Microplate Luminometer w/Dual Injectors . For more information please visit us at: https://www. Enter the Pub Hub and connect to the resources you need to answer your questions, find about the latest products and applications or laugh at the latest cartoon. Quantitative PCR (qPCR) (user operated) For usage prices please see our Rates page. Ltd. In addition to high performance, the GloMax® 20/20 blends user-friendly operation and a small footprint with flexible purchasing options. Statistical Endoproteinase Lys-C is a highly-purified serine protease isolated from Lysobacter enzymogenes that hydrolyzes specifically at the carboxyl side of Lysine. Engagement of TCR/CD3 on the cell surface leads to intracellular signaling events and the activation of nuclear transcription factors such as Nuclear Factor of Activated T cells (NFAT). Mar 16, 2010 · This is a short video that explains the proper usage of a luminometer. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. GloMax®-Multi Jr Accessories. This video shows you how to pronounce Luminometer. They are used for many different applications including assessing toxicity of drug candidates and other compounds, analyzing cellular changes in cancer and other diseases, and studying basic cell biology. Figure 3. This instrument has passed validation at Promega Corporation to be certified as DLReady. We therefore gathered salient information about many of the instruments available to date Promega GloMax Multiplus Plate Reader (flourescence/UV/absorbance)/Luminometer (dual injectors) user-operated) Promega 20/20 Single-Tube Luminometer (user-operated) Nanodrop ND-1000 and 2000c Spectrophotometers (user-operated) Qubit 2. It can be equipped with up to four reagent injectors and temperature control. T cell activation is initiated by engagement of the T cell antigen receptor (TCR)/CD3 complex and the co-stimulatory receptor CD28. Useful for a variety of luminometric research applications, the Thermo Scientific Luminoskan Microplate Luminometer is a compact and robust instrument offering plate format versatility, fast reading speeds, and up to three dispensers. TOP > Products > Luminometer. Besides LacZ, CAT and GFP, are there other alternatives which does not need a luminometer Utilizing a Novel Engineered Shrimp-Derived Luciferase to Enable Real-Time In Vitro Measurement of Cell Viability and Apoptosis using a Plate-Reading Luminometer The program will be presented by Terry Riss, PhD, Global Strategic Manager Cell Health at Promega Corporation Find out all of the information about the Promega France product: tube luminometer GloMax® 20/20. Transcriptional regulation, coupled to the expression of a luciferase reporter gene, The 20/20 n Luminometer is an ultrasensitive, versatile and affordable luminometer designed for use with Promega bioluminescent assays for reporter genes, kinases, caspases, p450, cell viability and more. A luminometer is an instrument that measures weak emissions of visible light coming from a sample, by means of a photomultiplier tube. Analytical & Material Science The clinical features and immune responses of asymptomatic individuals infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have not been well described. The Turner BioSystems TD-20/20 Luminometer combined with the Promega ENLITEN® ATP Assay System Bioluminescence Detection Kit provides a sensitive, rapid method for measuring adenosine 5'-triphosphate (ATP). Luciferase is the most widely used genetic reporter in studies on gene expression due to it’s high sensitivity dynamic range and its natural absence from mammalian cells. INTRODUCTION The Turner BioSystems' Veritas™ Microplate Luminometer in combination with the Steady-Glo® Assay kit provides a convenient, rapid, and sensitive procedure for quantifying gene expression. GL-220: Standard type / GL-220A: High-sensitivity type. High performance & Multi-function Equipped with RLU, dual-luciferase assay, kinetic-mode. We are an ISO 13485 certified company. Promega Corporation ·2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526· Phone 608-274-4330 Fax 608-277-2516 · · www. The light output from the system is extremely bright but has a half-life of <10 minutes. KG, Thermo Fisher Scientific, Meril Life Sciences Pvt. the regulation of its expression. The Reporter™Microplate Luminometer, combined with Promega’s Bright-Glo™ Luciferase Assay System, provides an easy to use, sensitive method in the quantitation of gene expression. Quantus comes preprogrammed with protocols for quantification of single-stranded DNA, double-stranded DNA and RNA using Promega QuantiFluor® Dyes and is also compatible with other fluorescent assays. CellTiter-Glo® 2. ZERO BIAS - scores, article reviews, protocol conditions and more Promega glomax 96 microplate luminometer Glomax 96 Microplate Luminometer, supplied by Promega, used in various techniques. Flexible speed and amplitude settings allow easy adaptation to any assay protocol. This guide compares microplate readers, luminometer and  The GloMax® 20/20 (single-tube) Luminometer is popular with labs that have low throughput needs. It is the instrument of choice for Reporter Gene Assays and ATP People usually have a promoter/luciferase gene construct and read luciferase activity with a luminometer. Transcriptional regulation, coupled to the A luminometer is an instrument capable of detecting photons of light that are emitted from either a chemical or enzymatic reaction. ATP is quantified by measuring the light produced through its reaction with the naturally-occurring firefly enzyme luciferase using a luminometer. The microplate is placed in a light-tight read chamber, and light from each well is detected in turn by a PMT. Bioz Stars score: 93/100, based on 5556 PubMed citations. It is a versatile system designed to perform both flash- and glow-type luminescence assays, with superior sensitivity and precision for all luminescent assays. The GloMax 20/20 Luminometer combines instrumentation and software in a complete solution that includes bioluminescent assays, protocols and support. A luminometer can be used for a wide variety of assays including luciferase assays, ATP assays, cellular luminescence, gene reporter assays, enzyme activity assays, and cell-based assays for Promega GloMax 20/20 Luminometer. Applications. The Genomics Core offers workshops in qPCR theory and practice multiple times per year. Promega Glomax 20/20 Luminometer The GloMax® 20/20 Luminometer is an ultrasensitive, compact laboratory instrument for measuring luminescent materials. All GloMax® microplate readers are designed to work seamlessly with Promega luminescence- and fluorescence-based assays: GloMax® Discover is a microplate reader with luminescence, fluorescence, BRET/FRET and UV-Visible absorbance detection capabilities. Instructions for Use of Product(s) E3651. After that, you will need to contact Customer Service to unlock your account. The GloMax® 96 Microplate Luminometer in combination with the Caspase-Glo® Assay provides a convenient procedure for measuring caspase-3 or -7 activities. The Wizard® Genomic DNA Purification Kit is designed for isolation of DNA from white blood cells, tissue culture cells and animal tissue,plant tissue, yeast, Gram-positive and Gram-negative bacteria. The GloMax® 96 Microplate Luminometer provides high sensitivity and broad dynamic range for all luminescence applications. ZERO BIAS - scores, article reviews, protocol conditions and more The Kinase-Glo luminescent kinase assay (Promega) is a high-throughput method for detecting protein kinase activity by quantifying the amount of ATP remaining following a kinase reaction. In this study we report the characterization of a new recombinant Asp-specific protease, rAsp-N, and utilize the NISTmAb reference IgG to develop protocols for efficient digestion and improved sequence coverage compared to trypsin alone. Every Veritas™ Luminometer also includes a power cable, data cable, Quick Start Guide, 5 white 96-well microplates, and software required to operate the instrument. # 27-1006) Promega luminometers include microplate and single-tube luminescence detection systems optimized for use with Promega assay chemistries. Multiplexing assays in the same sample well allows complementary measures of cell health to correct for errors in culturing, plating or assay chemistry interferences. The touchscreen on the luminometer allows easy setup and operation. We therefore gathered salient information about many of the instruments available to date Luminometers Note: It is suggested to use a dedicated luminometer when running MycoAlert™ Assays. The winner was a 1975 LKB Wallac 1250 Luminometer that was still in use at the University of Milan in Italy (Figure ). You also get a useful "Molecular Biology Lab Guide", your personal tutor to explain basic concepts in molecular biology research and a handy Promega coffee mug. Suitable for various applications Compact, Easy-to-use, Reasonable luminometer. The name was first used by Raphaël Dubois who invented the words luciferin and luciferase, for the substrate and enzyme , respectively. Promega SOT 2020 Virtual Booth mai 13, 2020–juin 30, 2020 Because we won’t be able to meet you in person at our booth this year, we’re bringing our booth to you. 5X10-15 moles ATP using CellTiter-Glo™ Substrate. Transcriptional regulation, coupled to the expression of a reporter gene, is regularly used to study a wide range of physiological events. Transcription of the gene encoding sterol regulatory element-binding protein 1c (SREBP-1c) is known to be activated by insulin in the liver. The linear range of the luminometer used for the assay must be established. Buy "GloMax 96 Microplate Luminometer - Reporter Assays, Promega - Model PAE6501 - Each" on Amazon. GloMax® Discover is a ready-to-use multimode plate reader developed with Promega reagent chemistries to provide a simple means of detecting luminescence, fluorescence and absorbance. Orion II features the highest sensitivity thanks to highly efficient optics and photon counting mode. E. Four convenient product packages valued up to 5000 SEK (or equivalent value in local currency). com Part# TM040 Printed in USA. Interior view of the GloMax® 96 Microplate Luminometer. Promega luminometers include microplate and single-tube luminescence detection systems optimized for use with Promega assay chemistries. The instrument comes ready to use—simply unpack it, plug it in and begin your experiments. John associates ECHA Microbiology Nokeval BioThema 3M Microtec A 20/20n Luminometer Method for Promega Bright-Glo™ 1. P. The GloMax®-20/20 Luminometer Light Standard provides an external control to verify performance. Glomax 20/20 Luminometer. growth media from untransfected cells) should be included in the assay to establish the background signal in the assay. The GloMax® 96 Microplate Luminometer is an easy-to-use, highly sensitive microplate luminometer with a broad dynamic range. The LUMIstar OPTIMA offers high performance luminescence (flash and glow) and BRET capabilities and has additional advanced features including top and bottom reading, incubation up to 45°C or 60°C, shaking, up to two on-board injectors, gas vent connection as well as BMG LABTECH’s established Reader Contro ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is converted into light by Ultra-Glo™ Luciferase. Figure 1. Page 6 Revised 3/12 5488MA These accessories are only for the now-discontinued Turner BioSystems Luminometer Model TD-20/20. Manufacturer: Promega; DESCRIPTION The GloMax 20/20 Luminometer is an ultrasensitive, versatile and affordable luminometer designed for use with any Promega bioluminescent assay. I need to measure ATP on luminometer with luciferase-luciferin assay. Not for use in diagnostic or therapeutic procedures. The resultant SREBP-1c protein activates transcription of the genes required for fatty acid synthesis. As well as includes different business approaches preferred by the decision-makers. Luminometer Cuvettes 12X75mm. Rear view of the GloMax® 96 Microplate Luminometer. Instructions for Use of Product(s) E1910, E1960. Instructions for Use of Product(s) A1120, A1125, A1620. GM2000, GM2010. We have 1 Promega GloMax GM2000 manual available for free PDF download: Operating Manual The main purpose of the reporter gene assay is to investigate the promoter of a gene of interest, i. Cell lysis occurs following the addition of Caspase-Glo® 3/7 Reagent. You can also interpret results using integrated data analysis software. Some of the luminometers manufacturers include Promega corporation, Thermo Fisher Scientific, Berthold Technologies, BMG labtech, HORIBA Scientific, Molecular Devices, LLC, and 3M. The samples were then analyzed using a luminometer. The new Orion L Microplate Luminometer is a compact, very affordable and user-friendly instrument, which uses high quality materials and advanced technologies. com TM278 · Revised 1/16 Fluorometers The Quantus™ Fluorometer is an easy-to-use, affordable single-tube fluorometer. I perform my transfections in 24-well plates and perform the assay as outlined by Promega (100 µl lysis and use 20µl of extract + 100 µl reagent and 1-second integration time). Literature # TM050. 5 µL of RNasin Plus (Promega), and 25 nM Z0P target DNA (Table S1). Figure 4. For measuring fluorescence and luminescence. eastport. Promega Absorbance Module for GloMax™-Multi Jr Single-Tube Multimode Reader The GloMax-Multi Jr Single-Tube Multimode Reader is designed to provide the utmost flexibility. Promega 20/20 Single-Tube Luminometer (user operated) For pricing see our Rates page. Global Microplate Luminometer Market To protect your privacy, your account will be locked after 6 failed attempts. com Part# TM278 Printed in USA. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 5 www. 2 Mag 20/20 Technology, Inc. Search. Promega offers an extensive toolbox of reporter bioassays to characterize and develop novel monoclonal antibody (mAb) and cell-based therapeutics. cz. Select Country. Let’s start by discussing two types of luciferase assays: “flash” vs. com Part# TM045 Printed in USA. Oct 17, 2019 · Abstract: The primary objective of the ‘Global Luminometers Market 2020-2029‘ research is to describe the complete characteristics of the industry, size of the various segments and the geographies as well as to forecast the trends and market statistics that are inclined to build up traction in the upcoming years. com FREE SHIPPING on qualified orders May 28, 2020 · The MarketWatch News Department was not involved in the creation of this content. Bioz Stars score: 94/100, based on 34 PubMed citations. Duplicate 25 µL translations were carried out in Flexi-Rabbit Reticulocyte Lysate (Promega) according to the manufacturer’s protocol using 2 pmol of each mRNA encoding for PBSII-NFluc (residues 2-416) and CFluc-Zif268 (residues 398-550), 10 µM S4 ZnCl2, 0. Promega GloMax Multiplus Plate Reader (user operated) fluorescence ; absorbance ; luminometer; dual injectors; For prices see our Rates page. The Microplate Luminometer market study is based on historical information and present market requirements. Promega Glomax 20/20 Luminometer #44752203. 96-well plate for luminometer (Corning #3912 white polystyrene flat bottom) Luminometer (we have a Molecular Devices Lmax luminometer) Reagents: cell culture media, 1xPBS, 1xTrypsin, G418 (200µg/µl) DMSO (Sigma D-5879) Bright-Glo Luciferase Assay reagent (Promega E2620) Drugs (we obtained the drugs as 100µg lyophilized on 96-well plates) The Promega Pub Hub is your online place for articles about new technologies and products, applications of existing products, tips, FAQspeak and LabFacts. There is good correlation between smoke point (Test Method D 1322) and luminometer number which is Buy "GloMax 96 Microplate Luminometer with Single Reagent Injector - Reporter Assays, Promega" on Amazon. Promega glomax 96 microplate luminometer Glomax 96 Microplate Luminometer, supplied by Promega, used in various techniques. Turn on luminometer and computer, open “Bright-Glo” program and edit template as desired. Více informací na www. May 28, 2020 (Profound via COMTEX) -- Microplate Luminometer Market 2020 Report offers a professional and in-depth • luminometer, CCD camera or imaging device capable of reading luminescence in multiwell plates • optional: ATP for use in generating a standard curve (see Section 3. Актуальные цены, клиентская поддержка, сервис Promega планшетный люминометр glomax  Promega Thermal Serial Printer and Universal Power Cable. Bioz Stars score: 93/100, based on 92 PubMed citations. The GloMax® 96 is a state-of-the-art microplate luminometer that meets the needs for high sensitivity and broad dynamic range for all luminescence applications. This advanced luminometer Some of the major players identified in the global microplate luminometer market are Promega Corporation, Tecan Trading AG, Danaher Corporation, Berthold Technologies GmbH & Co. A protocol to determine the number of viable cells in culture based on quantitation of the ATP present, which signals the presence of metabolically active cells. 5ml 35mm GloMaxTM 20/20n Luminometer PMT (photon-counting photomultiplier tube) S/N 700 8 8 Promega Luciferase Assay on the GloMaxTM 20/20n Firefly Luciferase (moles) The TD-20/20 Luminometer was designed by Turner BioSys- tems and Promega to run Promega's genetic reporter and ATP assays as well as other luminescence assays used in research lab- oratories. Luminometers A luminometer is an instrument used to measures light and other optical properties of specimens in chemiluminescent and bioluminescent applications. Here, we use SREBP-1c promoter reporter constructs to dissect the mechanism of insulin activation in freshly isolated rat hepatocytes. Dispense 20 mL cell lysate into white opaque luminometer microtiter plate Prime P injector (7 injections) with LARII Inject 100 ml LARII per well with P-injector. Day 3: Assay for luciferase activity with Promega Bright-Glo reagent Luciferase assay: 1. This assay is designed for use in automated high-throughput screening (HTS), cell proliferation and cytotoxicity assays. The data show In the present study, we aimed to decipher the mechanisms involved in the transcriptional effect of insulin on the SREBP-1c specific promoter of the human srebf-1 gene. Cell Lysis 1. thelabworldgroup. Contact a supplier or the parent company directly to get a quote or to find out a price or your closest point of sale. PLoS ONE Alerts: New Articles. For plate assays, the Promega GloMax Multiplus Plate Reader/Luminometer is the best choice. Brands : PROMEGA Mar 03, 2015 · Promega GloMax 96 Microplate Luminometer Model 9101-002 (Turner Biosystems) (Lot 342) luciferase for genetic reporter studies and ATP-based biomass analysis, and so is an ideal platform for reading Promega bioluminescent assays. Shop a large selection of Promega Turner Designs Luminometer Model TD-20/20 Accessories products and learn more about Promega Turner Designs Luminometer Model TD-20/20 using the Promega ENLITEN® ATP Assay: Up to 6 orders of magnitude dynamic range using the Promega ENLITEN® ATP Assay Minimum Detection Limit: 5x10-19 moles (0. Lys-C retains proteolytic activity under protein denaturing conditions such as 8M urea, which is used to improve digestion of proteolytically resistant proteins. Dedicated microplate luminometer. Promega Corporation offers technical support and solutions to the life sciences industry. Tube luminometers, compared to microplate luminometers, are very affordable and offer excellent sensitivity and reliability, but have a lower throughput and flexibility than plate luminometers. In addition to high performance, the GloMax-Multi Jr blends user-friendly operation and a small footprint with flexible purchasing options. Manufacturer: Promega; Promega Glomax 20/20 Luminometer The GloMax® 20/20 Luminometer is an ultrasensitive, compact laboratory instrument for measuring luminescent materials. The GloMax 96 Microplate Luminometer is an easy-to-use, highly sensitive microplate luminometer with a broad dynamic range. The GloMax® 20/20 Luminometer is designed to provide ultra-high performance for bioluminescent and chemiluminescent assays . Disposable Polypropylene Test Tubes (8 × 50mm) — 1,000/pack; Star® Printer Paper; Interface Tubing and Valves for Auto Injector System Luminescence Detection A luminometer is a sensitive photometer designed to measure all bioluminescent and chemiluminescent assays. glomax luminometer Wetlands, Water Quality and Rapid Assays provided that full and clear credit is given to Promega Corporation with appropriate and specific Promega™ GloMax™ 96 Microplate Luminometer Light Plate Purchase up to $1,000 in Promega reagents and get a matching amount of products at no additional cost. Introducing the Lu-mini, a robust, single-tube, benchtop luminometer which can be used for a wide variety of assays including, luciferase assays, ATP assays, cell-based assays for drug discovery and many more. Promega Corporation •2800 Woods Hollow Road • Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 • Phone 608-274-4330 • Fax 608-277-2516 • www. Luminescence was measured on a Promega Glomax® 20/20 single tube luminometer with integration time of 1 second. Compact luminometer: Gene Light. Many will scan for fluorescence in addition to measuring UV and visible light absorbance. # 27-1006) Luminometers and Fluorometers Date: November 24, 1997 Chart To most people, a luminometer is something that probably measures "luminos," while a fluorometer measures "fluoros," As simple as that sounds, these analyzers have been occupying valuable bench space in laboratories for decades. The touch screen interface provides comprehensive instrument control and data collection. These accessories are not for the Turner BioSystems 20/20n Single-Tube Luminometer or the Promega™ GloMax™ 20/20 Luminometer. Detailed perspective towards Luminometer industry trends and drivers offer a clear overview how the Luminometer industry increasing during the slated period from 2020 to 2026. INTRODUCTION The Turner BioSystems' Veritas™ Microplate Luminometer in combination with Promega's Dual-Glo™ Luciferase Assay kit provides a convenient, rapid, and sensitive procedure for quantifying gene expression. Dec 10, 2019 · Dependent on which luciferase assay you use, you may need a luminometer that can use injectors to deliver the assay reagents. The built-in USB port is an added feature allowing lab users to keep up with ever changing PC demands. Background from reagents without enzyme added was subtracted from luminescence values. UV 2 DNA, RNA, 18 1. Literature # 9PIE365. GloMax 96 Microplate Luminometer is a compact, easy to use, low cost luminometer which meets the need of all chemiluminescent and bioluminescent applications. May 01, 2012 · Recently, Promega (Promega Corporation, Madison, WI, USA) organized a competition to find the oldest working luminometer in Europe and the 47 entries are displayed in an online photo‐gallery . Luciferase is a generic term for the class of oxidative enzymes that produce bioluminescence, and is usually distinguished from a photoprotein. To reserve time please go to our Reservation and Billing site. Thaw assay buffer in room temperature water. For measuring luminescence. 38[luc2P/p53 RE/Hygro] Vector contains two copies of a p53 response element (p53 RE) that drives transcription of the luciferase reporter gene luc2P (Photinus pyralis). 11 Luminometer and Microplate Reader Selection Guide From advanced multimode luminescence, fluorescence, absorbance, BRET and FRET capabilities to dedicated microplate or single-tube luminometers, there is a GloMax® system for every lab and throughput need. Suitable for various applications. # 27-1006) May 29, 2015 · This video shows you how to pronounce Luminometer. microplate luminometer. Additional outstanding features of the LUMIstar Omega include top and bottom reading, incubation up to 45°C or 65°C, up to two “smart” reagent injectors, gas vent connec Microplate Luminometer, supplied by Berthold Technologies, used in various techniques. Sep 15, 2014 · Dependent on which luciferase assay you use, you may need a luminometer that can use injectors to deliver the assay reagents. Using luciferase reporter gene constructs in HEK-293 cells (human embryonic kidney cells), we demonstrated that the full effect of insulin requires the presence of SREs (sterol response elements) in the proximal region of the Jun 14, 2020 · Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous pollutants that are metabolized to carcinogenic dihydrodiol epoxides (PAHDE) by cytochrome P450… The Role of an Inverted CCAAT Element in Transcriptional Activation of the Human DNA Topoisomerase IIaGene by Heat Shock* (Received for publication, October 30, 1997) Jun 22, 2020 · By acting as a ligand-dependent transcription factor the glucocorticoid receptor (GR) mediates the actions of glucocorticoids and regulates many physi… Promega Tecan Berthold Technologies Thermo Fisher Scientific Meril Life Sciences Luminex SAFAS MicroDigital Danaher CTK Biotech Titertek-Berthold MICRODIGITAL. For use with the discontinued GloMax®-Multi Jr Single-Tube Multimode Reader. We have luminometer Fluoroscan Ascent FL, but when I change to luminometer mode, I have menu for filter… and there is writen NONE. I used a manual luminometer to analyze my sample The luciferase activity was measured by the addition of 20 µL of lysate to 100 µL of luciferase assay reagent II (Promega) in a 5 mL test tube followed by reading on a Berthold Lumat LB9501 luminometer. 0 Assay can be multiplexed with other assays, reducing cell culture costs and labor. INTRODUCTION The Turner BioSystems' 20/20n Luminometer in combination with Promega’s Bright-Glo™ Assay kit provides a convenient, rapid, and sensitive procedure for quantifying gene expression. A protocol for quantitative analysis of factors that may regulate mammalian gene expression, such as <i>cis</i>-acting factors like promoters and enhancers, or <;i>trans&lt;/i>-acting factors, like DNA-binding factors. The luciferase assay is extremely rapid, simple, relatively inexpensive, sensitive, and possesses a broad linear range. They are distributed in some viruses and… Automated methods for use of Promega reagents on the epMotion® 5075 VAC workstation RPL38 Regulates the Proliferation and Apoptosis of Gastric Cancer via miR-374b-5p/VEGF Signal Pathway Promega luminometers include microplate and single-tube luminescence detection systems optimized for use with Promega assay chemistries. A dual-masking system minimizes well-to-well cross-talk. Apr 04, 2011 · Luminometry Glomax od Promegy se vyznačují excelentním dynamickým rozsahem, citlivostí a flexibilitou co se týče možnosti použití formátu vzorků. This type of instrument can be a very useful piece of equipment for a variety of laboratories, including life science research, clinical, and applied areas such as food or water quality testing. It provides sensitivity down to 1 x 10-21 moles luciferase  Promega Corporation logo. This instrument can used by customers to run assays in a plate format (6, 12, 24, 48, 96 and 384-well). com TM493 · 3/17 2. Literature # TM278. Promega GloMax 96 Microplate luminometer The GloMax® 96 Microplate Luminometer represents instrumentation and software in a complete solution that includes bioluminescent assays, protocols and support. To fulfil this need, Promega developed a kinase assay that is based on luminescence and the by-product ADP. Details use of Promega’s Dual Luciferase Reporter Assay System with the Optocomp II luminometer. Bioluminescent reporter assays are an excellent choice for analyzing gene regulation because they provide higher sensitivity, wider dynamic range and better  дистрибьютор производителя Promega: люминометры . GloMax® 96 Microplate Luminometer Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 1 www. Chemiluminescence is the generation of electromagnetic radiation as ultraviolet, visible or infrared light by the release of energy from a chemical reaction. Home; About Us; Products . GloMax® Navigator is an easy-to-use microplate luminometer with superior luminescence detection capabilities. Our products help scientists who are engaged in research in life scienc more EMAIL INQUIRY to 3 Luminometer Light Standards suppliers Promega Corporation •2800 Woods Hollow Road • Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 • Phone 608-274-4330 • Fax 608-277-2516 • www. 4. The user interface, sample compartment and injectors are all optimized for easy, sensitive, and accurate dual luciferase and ATP assays. To reserve time go to our Reservation and Billing web site. In the Caspase-Glo® Assay a proluminescent caspse-3/7 substrate contains the signature DEVD peptide sequence. Tube luminometers are the instrument of choice for laboratories with low throughput or a limited budget. # 27-1006) This sensitive instrument is used to measure luminescence. β-Galactosidase activity was measured using chlorophenol red-β-d-galactopyranoside (CPRG) (Sigma-Aldrich, St. High-performance microplate reader for detecting luminescence, fluorescence and absorbance. Reagents can be connected easily, and the low dead volume 泽浩公司是云南昆明专业实验室产品供应商,涵盖抗体,试剂,耗材,仪器,是Promega,BI,STEMCELL,ACDbio,BioGems,peprotech,proteintech云南总代理 Promega GloMax Luminometers are supplied with preinstalled protocols that allow researchers to perform multiplex bioluminescent assays. Luminometers are very sensitive devices that are used to measure very small amounts of light, sometimes only a handful of photons. 5 amol ATP per well or 1. The Dual-Luciferase® Reporter (DLR™) Assay System provides an efficient means of performing dual-reporter assays. Promega Benelux is proud to present its first funding opportunities for young academic researchers working in the field of Oncology and those who are taking their first steps in this interesting research field. Turner BioSystems 20/20n Single-Tube LuminometerまたはPromega GloMax® 20/20 Luminometerでは使用できません。 プロメガは、シングルチューブ&96ウェルプレート解析用の次世代ルミノメータの開発において更なる開発を進めています。 Luminometer. This instrument has passed the DLReady™ validation at Promega Corporation™ and is certified as DLReady. That enhanced the Microplate Luminometer industry growth and make a phenomenal stand in the industry. Maximize your bench space with our compact, lightweight and low-cost luminometer. The system generates both firefly and Renilla luciferase luminsecence signals from cells that have n Seminar : Utilizing a Novel Engineered Shrimp-Derived Luciferase to Enable Real-Time In Vitro Measurement of Cell Viability and Apoptosis using a Plate-Reading Luminometer BioVille Promega en BioVille nodigen je graag uit voor een interessant seminarie over celviabiliteit en apoptose: A protocol for a vector containing a cAMP response element that drives the transcription of the luciferase reporter gene luc2P, which contains hPEST, a protein destabilization sequence. Lonza Luminometers Instrument MycoAlert™ Assay MycoAlert™ PLUS Assay Other Bioassays* Notes Quantilum® Luciferase (Promega) was serially diluted in 1X Firefly Lysis Buffer with 1 mg/mL BSA and measured in the assay. Promega's CellTiter-Glo™ Assay on the Veritas™ Microplate Luminometer using ATP standard diluted in HEPES buffer. A luminometer for assaying bioluminescence and chemiluminescence reactions characterized by sample wells having transparent bottom walls, a light detector positioned beneath the sample wells and a reagent injector above the wells. 5 amol ATP per well or <1. This plate reading luminometer is optimized with for use with Promega cell and gene reporter assays and comes with over 30 preloaded assay protocols. Add assay buffer to lyophilized substrate. We Have a Solution for You. I performed the assay exactly described in the protocol,and detected the luminescence with our Luminoskan Ascent luminometer. The pGL4. These assays are useful for interrogating a range of biological functions, including Fc effector activity, immune checkpoint modulation, T cell activation, and cytokine and growth factor signaling. These packages will let you test your skills in cloning, DNA / RNA work or cellular analysis. The Orion II is more than a basic luminometer. The LUMIstar Omega is also DLReadyTM certified from Promega. The GloMax® line includes high-performance plate readers with a range of detection options. Promega is a global leader in providing innovative solutions and technical support to life scientists in academic, industrial and government settings. A Veritas™ Microplate Luminometer Method for Promega Steady-Glo® 1. This can be done by linking the promoter of interest to an easily detectable gene, such as the gene for firefly luciferase, which catalyses a reaction that produces light. The GloMax® 20/20 Luminometer is designed to provide ultra-high performance for bioluminescent and chemiluminescent assays. It is a state-of-the-art instrument offering complete configuration flexibility. It can be used for both short and long term stability check of the detection system. luminometer promega

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